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Oils obtained from olive pastes treated with 8 minute ultrasound improved Uslu oil yield 1.74% and the oil extractability 10.32%. Different sonication and malaxation time combinations did not induce difference (P˃0.05) in the Edremit oil yield and extractability indexes However, they were significantly different in Gemlik and Uslu oils. The extraction yield, free acidity, peroxide value, p-anisidin value, total oxidation value, K232 and K270, total phenol compound, the content of total chlorophyll and total carotenoid compounds were determined. In this study, the influence of malaxation time combined by ultrasound on the extraction yield, oxidative and quality characteristics of Extra Virgin Olive Oils extracted from of Edremit, Gemlik and Uslu cultivars were studied. Select a residue to mutate to from the drop down list or select "all" to try all 20 natural amino acids. If you have selected a residue it should be reported in the "Residue to Mutate" box. Step 2 Choose the option MolMechanics/Try Mutation/ Protein Stability and a dialog box as shown above will be displayed. Step 1 Read in the PDB file of interest and select the residue you wish to mutate. A positive energy value indicates that the mutation is likely to be destabilizing. The rest of the protein structure is considered rigid. Mutation of a given residue is followed by Monte Carlo simulations with flexible side chains for the mutated residue and its neighboring residues. The residue-specific energies were derived empirically using a large set of experimental data. The free energy change in protein stability is computed as follows:ĬalculationThe free energy of the unfolded and misfolded states is approximated by a sum of the residue-specific energies. "Scan Sequence" allows you to mutate all the residues in the sequence to the specified amino acid. 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The algorithm highlights the continued importance of non-pharmacological interventions. ![]() First published by Yale University Press in 1981, it examines how a number of seemingly insignificant events in 1587 might have caused the downfall of the Ming dynasty. New Haven and London, Yale University Press, 1981. ![]() 1587, a Year of No Significance: The Ming Dynasty in Decline (Chinese: 萬曆十五年 pinyin: Wanli Shiwunian) is Chinese historian Ray Huang's most famous work. 1587, a year of no significance: The Ming Dynasty in Decline. |